anti lc3 i ii (Proteintech)
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Anti Lc3 I Ii, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lc3+i+ii/LC3+Antibody/pm42767428-127-6-10
Average 96 stars, based on 2126 article reviews
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Blocking Assay:Article Title: Identification of ferroptosis-related genes as diagnostic biomarkers and therapeutic targets in sorafenib-resistant HCC via machine learning and experimental validation. Article Snippet: Identification of ferroptosis-related genes as diagnostic biomarkers and therapeutic targets in sorafenib-resistant HCC via machine learning and experimental validation Jinrui Zhang 1, Wenhan Geng 1, Haojin Sun 1, Jian Chen 1, Yumei Fan *, Ke Tan * Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaborative Innovation Center for Eco-Environment, Hebei Province Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, China A R T I C L E I N F O Editor: Jean-Pierre GORVEL Keywords: sorafenib resistant ferroptosis diagnosis machine learning algorithms A B S T R A C T Liver cancer, particularly hepatocellular carcinoma (HCC), remains a significant health burden with poor survival rates due to late diagnosis and limited treatment options.. Sorafenib, a multi-kinase inhibitor, is the standard first-line therapy for advanced HCC, but long-term use often leads to drug resistance.. Ferroptosis, an irondependent form of regulated cell death, has emerged as a crucial target in cancer therapy, showing potential to enhance treatment efficacy and overcome drug resistance. Incubation:Article Title: Identification of ferroptosis-related genes as diagnostic biomarkers and therapeutic targets in sorafenib-resistant HCC via machine learning and experimental validation. Article Snippet: Identification of ferroptosis-related genes as diagnostic biomarkers and therapeutic targets in sorafenib-resistant HCC via machine learning and experimental validation Jinrui Zhang 1, Wenhan Geng 1, Haojin Sun 1, Jian Chen 1, Yumei Fan *, Ke Tan * Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaborative Innovation Center for Eco-Environment, Hebei Province Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, China A R T I C L E I N F O Editor: Jean-Pierre GORVEL Keywords: sorafenib resistant ferroptosis diagnosis machine learning algorithms A B S T R A C T Liver cancer, particularly hepatocellular carcinoma (HCC), remains a significant health burden with poor survival rates due to late diagnosis and limited treatment options.. Sorafenib, a multi-kinase inhibitor, is the standard first-line therapy for advanced HCC, but long-term use often leads to drug resistance.. Ferroptosis, an irondependent form of regulated cell death, has emerged as a crucial target in cancer therapy, showing potential to enhance treatment efficacy and overcome drug resistance. Article Title: Novel Isolongifolenone-Based Caprolactam Derivatives as Potential Anticancer Agents via the p53/mTOR/Autophagy Pathway. Article Snippet: .. The membranes were then blocked with BSA for 2 h at room temperature and incubated with primary antibodies against p-mTOR (ImmunoWay Biotechnology, Beijing, China, YP1444, 1:5000), P62 (Proteintech Group, Inc., Wuhan, China, 60268-1-IG, 1:5000), Article Title: Novel Isolongifolenone-Based Caprolactam Derivatives as Potential Anticancer Agents via the p53/mTOR/Autophagy Pathway Article Snippet: .. The membranes were then blocked with BSA for 2 h at room temperature and incubated with primary antibodies against p-mTOR (ImmunoWay Biotechnology, Beijing, China, YP1444, 1:5000), P62 (Proteintech Group, Inc., Wuhan, China, 60268-1-IG, 1:5000), Article Title: O-GlcNAcylation of YAP1 promotes lung transplant ischemia-reperfusion injury via binding to HIF1α transcription factor and activating autophagy and mitophagy Article Snippet: .. Afterward, the membrane underwent treatment with 5% skim milk powder at room temperature for 1 h. It was then incubated overnight at 4 °C with primary antibodies against YAP1 (Cat No. 13584-1-AP, 1:1500), p-YAP1 (Cat No. 80694-2-RR, 1:2000), HIF-1α (Cat No. 66730-1-Ig, 1:1000), CYR61 (No. 26689-1-AP, 1:1500), AREG (Cat No. 16036-1-AP, 1:2300), BIRC5 (Cat No. 10508-1-AP, 1:1200), FUNDC1 (Cat No. 28519-1-AP, 1:1500), PINK1 (Cat No. 23274-1-AP, 1:2000), TBK1 (Cat No. 28397-1-AP, 1:1500), HMGB1 (Cat No. 10829-1-AP, 1:1000), DAPK (Cat No. 25136-1-AP, 1:1000), Article Title: O-GlcNAcylation of YAP1 promotes lung transplant ischemia-reperfusion injury via binding to HIF1α transcription factor and activating autophagy and mitophagy. Article Snippet: .. It was then incubated overnight at 4 °C with primary antibodies against YAP1 (Cat No. 13584-1-AP, 1:1500), p-YAP1 (Cat No. 80694-2-RR, 1:2000), HIF-1α (Cat No. 66730-1-Ig, 1:1000), CYR61 (No. 26689-1-AP, 1:1500), AREG (Cat No. 16036-1-AP, 1:2300), BIRC5 (Cat No. 10508-1-AP, AR TI CL E IN P RE SS 1:1200), FUNDC1 (Cat No. 28519-1-AP, 1:1500), PINK1 (Cat No. 23274-1-AP, 1:2000), TBK1 (Cat No. 28397-1-AP, 1:1500), HMGB1 (Cat No. 10829-1-AP, 1:1000), DAPK (Cat No. 25136- 1-AP, 1:1000), other:Article Title: Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1. Article Snippet: The signals were visualized and imaged using the ChemiDoc imaging system (BioRad). Western Blot:Article Title: Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1 Article Snippet: The band intensity was quantified by ImageJ software (NIH) and normalized to the housekeeping gene that served as internal control. .. The antibodies for Western Blot and their dilution are listed as follows: USP35 (Proteintech, Cat#: 24559-1-AP, 1:1000), FUNDC1 (Proteintech, Cat#: 28519-1-AP, 1:2000), Cyt C (Proteintech, Cat#: 10993-1-AP, 1:1000), BAX (Proteintech, Cat#: 50599-2-Ig, 1:1000), BCL2 (Proteintech, Cat#: 12789-1-AP, 1:2000), total and cleaved Caspase 3 (Proteintech, Cat#: 19677-1-AP, 1:1000), total and cleaved Caspase 9 (Proteintech, Cat#: 10380-1-AP, 1:1000), Beclin-1 (Proteintech, Cat#: 11306-1-AP, 1:1000), p62 (Proteintech, Cat#: 18420-1-AP, 1:5000), Luciferase:Article Title: Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1 Article Snippet: The band intensity was quantified by ImageJ software (NIH) and normalized to the housekeeping gene that served as internal control. .. The antibodies for Western Blot and their dilution are listed as follows: USP35 (Proteintech, Cat#: 24559-1-AP, 1:1000), FUNDC1 (Proteintech, Cat#: 28519-1-AP, 1:2000), Cyt C (Proteintech, Cat#: 10993-1-AP, 1:1000), BAX (Proteintech, Cat#: 50599-2-Ig, 1:1000), BCL2 (Proteintech, Cat#: 12789-1-AP, 1:2000), total and cleaved Caspase 3 (Proteintech, Cat#: 19677-1-AP, 1:1000), total and cleaved Caspase 9 (Proteintech, Cat#: 10380-1-AP, 1:1000), Beclin-1 (Proteintech, Cat#: 11306-1-AP, 1:1000), p62 (Proteintech, Cat#: 18420-1-AP, 1:5000), Membrane:Article Title: O-GlcNAcylation of YAP1 promotes lung transplant ischemia-reperfusion injury via binding to HIF1α transcription factor and activating autophagy and mitophagy Article Snippet: .. Afterward, the membrane underwent treatment with 5% skim milk powder at room temperature for 1 h. It was then incubated overnight at 4 °C with primary antibodies against YAP1 (Cat No. 13584-1-AP, 1:1500), p-YAP1 (Cat No. 80694-2-RR, 1:2000), HIF-1α (Cat No. 66730-1-Ig, 1:1000), CYR61 (No. 26689-1-AP, 1:1500), AREG (Cat No. 16036-1-AP, 1:2300), BIRC5 (Cat No. 10508-1-AP, 1:1200), FUNDC1 (Cat No. 28519-1-AP, 1:1500), PINK1 (Cat No. 23274-1-AP, 1:2000), TBK1 (Cat No. 28397-1-AP, 1:1500), HMGB1 (Cat No. 10829-1-AP, 1:1000), DAPK (Cat No. 25136-1-AP, 1:1000), |

